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Human ABI1 (Abl Interactor 1) ELISA Kit

CAT: 0855-ELK4195-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4195-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABI1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
ABI-1; E3B1; NAP1BP; SSH3BP1; Spectrin SH3 Domain Binding Protein 1; Abelson interactor 1; Abl-binding protein 4; Nap1-binding protein; Eps8 SH3 domain-binding protein
UniProt
Q8IZP0
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABI1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.057 ng/mL

UniProtKB · Q8IZP0

Abl interactor 1

ABI1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8IZP0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ABI1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A9Z1Y6, B3KX62, B4DQ58, H7BXI6, O15147, O76049, O95060, Q5T2R3, Q5T2R4, Q5T2R6, Q5T2R7, Q5T2R9, Q5W070, Q5W072, Q8TB63, Q96S81, Q9NXZ9, Q9NYB8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicingChromosomal rearrangement

Cellular component

Cell projectionCytoplasmCytoskeletonNucleusSynapse

Domain

Coiled coilSH3 domain

Biological process

Host-virus interaction

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