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Human EMILIN2 (Elastin Microfibril Interface Located Protein 2) ELISA Kit

CAT: 0855-ELK4200-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4200-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EMILIN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMILIN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EMILIN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EMILIN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
FOAP-10; Elastin Microfibril Interfacer 2
UniProt
Q9BXX0
Reactivity
Human
Field of Research
CD & Adhesion molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EMILIN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EMILIN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EMILIN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EMILIN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.059 ng/mL

UniProtKB · Q9BXX0

EMILIN-2

EMIL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BXX0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EMILIN2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2RMY3, Q8NBH3, Q96JQ4
Protein keywords

Biological process

Cell adhesion

Domain

Coiled coilCollagenSignal

PTM

Disulfide bondGlycoprotein

Cellular component

Extracellular matrixSecreted

Technical term

Proteomics identificationReference proteome

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