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Mouse iNV (Involucrin) ELISA Kit

CAT: 0855-ELK4423-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4423-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse iNV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse iNV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse iNV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse iNV in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IVL
UniProt
P48997
Reactivity
Mouse
Field of Research
Apoptosis; Tumor immunity; Ophthalmology & Otorhinolaryngology; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse iNV. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse iNV. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse iNV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse iNV in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · P48997

Involucrin

INVO_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P48997
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ivl
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

Cytoplasm

Biological process

Keratinization

Technical term

Reference proteome

Domain

Repeat

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