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Human PRM1 (Protamine 1) ELISA Kit

CAT: 0855-ELK4763-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4763-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CT94.1; Cancer/Testis Antigen Family 94, Member 1; Sperm protamine P1; Cysteine-rich protamine
UniProt
P04553
Reactivity
Human
Field of Research
Reproductive science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.058 ng/mL

UniProtKB · P04553

Sperm protamine P1

HSP1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04553
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRM1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

ChromosomeNucleosome coreNucleus

Molecular function

Developmental proteinDNA-binding

Biological process

DifferentiationDNA condensationSpermatogenesis

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondPhosphoprotein

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