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Mouse DAT (Dopamine Transporter) ELISA Kit

CAT: 0855-ELK5272-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5272-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DAT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SLC6A3; DAT1; Sodium-dependent dopamine transporter; Dopamine Active Transporter; SLC6-A3; Solute Carrier Family 6 (neurotransmitter Transporter, Dopamine), Member 3
UniProt
Q61327
Reactivity
Mouse
Field of Research
Signal transduction; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DAT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
40 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.63-40 ng/mL
Sensitivity
0.26 ng/mL

UniProtKB · Q61327

Sodium-dependent dopamine transporter

SC6A3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61327
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Slc6a3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
DA transporter, DAT
EC number
—
Processing
—
Secondary accessions
Q60719, Q9R1I2
Protein keywords

Cellular component

Cell membraneCell projectionMembrane

PTM

Disulfide bondGlycoprotein

Ligand

Metal-bindingSodium

Biological process

Neurotransmitter transportSymportTransport

Technical term

Reference proteome

Domain

TransmembraneTransmembrane helix

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