Products for Research Use Only
RegisterLogin

Mouse CD8 (Cluster ofDifferentiation 8) ELISA Kit

CAT: 0855-ELK5327-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK5327-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
P32; CD8-A; Leu2; MAL; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; CD8a; Cluster Of Differentiation 8a
UniProt
P01731
Reactivity
Mouse
Field of Research
Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.064 ng/mL

UniProtKB · P01731

T-cell surface glycoprotein CD8 alpha chain

CD8A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01731
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd8a
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Biological process

Adaptive immunityImmunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Related Products

CatalogName
ELK7813-01Chicken CD8 (Cluster ofDifferentiation 8) ELISA Kit
ELK9501-01Rat CD8 (Cluster ofDifferentiation 8) ELISA Kit
ELK9807-01Horse CD8 (Cluster ofDifferentiation 8) ELISA Kit
ELK5104-01Human CD8 (Cluster ofDifferentiation 8) ELISA Kit
ELK5327MS-01Mouse CD8 (Cluster ofDifferentiation 8) Microsample ELISA Kit
ELK9501MS-01Rat CD8 (Cluster ofDifferentiation 8) Microsample ELISA Kit
ELK5104MS-01Human CD8 (Cluster ofDifferentiation 8) Microsample ELISA Kit
ELK3356-01Mouse CD83 (Cluster Of Differentiation 83) ELISA Kit
ELK10536-01Human CD83 (Cluster Of Differentiation 83) ELISA Kit
ELK7411-01Human CD8b (Cluster Of Differentiation 8b) ELISA Kit

Alternative Products

CatalogName
EK244808Mouse CD8 (Cluster ofDifferentiation 8) ELISA Kit
GTR18811226-01Mouse Cluster ofDifferentiation 8 (CD8) ELISA Kit
GTR18872432-01Mouse CD8 (Cluster ofDifferentiation 8) Microsample ELISA Kit
KLV0228GENLISA Equine Cluster Ofdifferentiation 8 (CD8) ELISA
MBS8820475-01Horse CD8 (Cluster ofDifferentiation 8) ELISA Kit
EK244592Human CD8 (Cluster ofDifferentiation 8) ELISA Kit
GTR18821413-01Horse Cluster ofDifferentiation 8 (CD8) ELISA Kit
GTR18819374-01Rat Cluster ofDifferentiation 8 (CD8) ELISA Kit
EK247242Chicken CD8 (Cluster ofDifferentiation 8) ELISA Kit
GTR18813366-01Chicken Cluster ofDifferentiation 8 (CD8) ELISA Kit

Popular Products