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Mouse PSPN (Persephin) ELISA Kit

CAT: 0855-ELK6232-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6232-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PSP
UniProt
O70300
Reactivity
Mouse
Field of Research
Cytokine; Infection immunity; Neuro science; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PSPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PSPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PSPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PSPN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
7.7 pg/mL

UniProtKB · O70300

Persephin

PSPN_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O70300
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pspn
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PSP
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Disulfide bond

Molecular function

Growth factor

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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