Human RCBTB2 (RCC1 And BTB Domain Containing Protein 2) ELISA Kit

Human RCBTB2 (RCC1 And BTB Domain Containing Protein 2) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RCBTB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RCBTB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RCBTB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RCBTB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CHC1L; RLG; RCC1-like G exchanging factor; Regulator Of Chromosome Condensation And BTB(POZ)Domain Containing Protein 2; Chromosome Condensation 1-Like
• UniProt: O95199
• Field of Research: Signal transduction.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 20 ng/mL
• Sample Type: Tissue homogenates and other biological fluids.
• Detection Range: 0.32-20 ng/mL
• Sensitivity: 0.124 ng/mL