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Mouse SDHA (Succinate Dehydrogenase Complex Subunit A) ELISA Kit

CAT: 0855-ELK7193-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7193-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
UniProt
Q8K2B3
Reactivity
Mouse
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.113 ng/mL

UniProtKB · Q8K2B3

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

SDHA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8K2B3
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sdha
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
1.3.5.1
Processing
Precursor
Secondary accessions
Q0QF19, Q3UH25, Q3UKP7, Q3V4B1, Q921P5, Q9Z1Z4
Protein keywords

PTM

AcetylationPhosphoprotein

Technical term

Direct protein sequencingReference proteome

Biological process

Electron transportTransportTricarboxylic acid cycle

Ligand

FADFlavoprotein

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Molecular function

Oxidoreductase

Domain

Transit peptide

Disease

Tumor suppressor

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