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Mouse SDHD (Succinate Dehydrogenase Complex Subunit D) ELISA Kit

CAT: 0855-ELK7195-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7195-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHD in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PGL; PGL1; QPs3; Succinate-ubiquinone reductase membrane anchor subunit; Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
UniProt
Q9CXV1
Reactivity
Mouse
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHD in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.067 ng/mL

UniProtKB · Q9CXV1

Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial

DHSD_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9CXV1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sdhd
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
CybS
EC number
—
Processing
Precursor
Secondary accessions
A6H629, Q3UX11
Protein keywords

Biological process

Electron transportTransportTricarboxylic acid cycle

Ligand

HemeIronMetal-binding

Cellular component

MembraneMitochondrionMitochondrion inner membrane

Technical term

Reference proteome

Domain

Transit peptideTransmembraneTransmembrane helix

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