Mouse BFSP1 (Beaded Filament Structural Protein 1) ELISA Kit

Mouse BFSP1 (Beaded Filament Structural Protein 1) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BFSP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BFSP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BFSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BFSP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: CP115; CP94; LIFL-H; Filensin; Lens fiber cell beaded-filament structural protein CP 115; Lens intermediate filament-like heavy
• UniProt: A2AMT1
• Field of Research: Signal transduction; Ophthalmology & Otorhinolaryngology; Developmental science.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.064 ng/mL