Rat LGALS3BP (Lectin Galactoside Binding, Soluble 3 Binding Protein) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LGALS3BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LGALS3BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LGALS3BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LGALS3BP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  MAC-2-BP; LGALS3-BP; Galectin-3-Binding Protein; L3 Antigen; Mac-2-Binding Protein; Tumor-associated antigen 90K; Serum Protein 90K; Basement membrane autoantigen p105

• Gene Aliases:

  MAC-2-BP; LGALS3-BP; Galectin-3-Binding Protein; L3 Antigen; Mac-2-Binding Protein; Tumor-associated antigen 90K; Serum Protein 90K; Basement membrane autoantigen p105

• UniProt:

  O70513

• Reactivity:

  Rat

• Field of Research:

  Tumor immunity, Infection immunity, Immunodeficiency

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LGALS3BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LGALS3BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LGALS3BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LGALS3BP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  100 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  1.57 - 100 ng/mL

• Sensitivity:

  0.67 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5