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Human MBL (Mannose Binding Lectin) ELISA Kit

CAT: 0855-ELK1098-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1098-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin (Protein C) 2, Soluble (Opsonic Defect)
UniProt
P11226
Reactivity
Human
Field of Research
Signal transduction; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL

UniProtKB · P11226

Mannose-binding protein C

MBL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P11226
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MBL2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MBP-C
EC number
—
Processing
Precursor
Secondary accessions
Q4VB12, Q4VB13, Q4VB14, Q5SQS3, Q86SI4, Q96KE4, Q96TF7, Q96TF8, Q96TF9
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Ligand

CalciumLectinMannose-bindingMetal-binding

Domain

Coiled coilCollagenRepeatSignal

Biological process

Complement activation lectin pathwayComplement pathwayImmunityInnate immunity

PTM

Disulfide bondHydroxylation

Cellular component

Secreted

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