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Mouse PRG4 (Proteoglycan 4) ELISA Kit

CAT: 0855-ELK7673-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7673-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRG4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MSF; CACP; HAPO; JCAP; SZP; Megakaryocyte Stimulating factor; Articular Superficial Zone Protein; Camptodactyly; Arthropathy, Coxa Vara, Pericarditis Syndrome
UniProt
Q9JM99
Reactivity
Mouse
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRG4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, synovial fluid and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.065 ng/mL

UniProtKB · Q9JM99

Proteoglycan 4

PRG4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9JM99
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prg4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q3UEL1, Q3V198
Protein keywords

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinProteoglycan

Technical term

Reference proteome

Domain

RepeatSignal

Cellular component

Secreted

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