Pig pPLA2 (Phospholipase A2, Pancreas) ELISA Kit

Pig pPLA2 (Phospholipase A2, Pancreas) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig pPLA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig pPLA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig pPLA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig pPLA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: PLA2G1B; PLA2A; PPLA2; Phospholipase A2, Group IB; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
• Field of Research: Enzyme & Kinase.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 2000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 31.25-2000 pg/mL
• Sensitivity: 13.5 pg/mL