IP3 (Inositol Triphosphate) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

• Gene Aliases:

  InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol

• Reactivity:

  General

• Field of Research:

  Signal transduction, Metabolic pathway, Endocrinology, Cardiovascular biology, Neuro science, Hormone metabolism, Bone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  10000 pg/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  156.25 - 10000 pg/mL

• Sensitivity:

  48.6 pg/mL

• Species:

  General

• CAS Number:

  7732-18-5