Pig IP3 (Inositol Triphosphate) ELISA Kit

Pig IP3 (Inositol Triphosphate) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pig IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol
• Field of Research: Signal transduction; Metabolic pathway; Endocrinology; Cardiovascular biology; Neuro science; Hormone metabolism; Bone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 10000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 156.25-10000 pg/mL
• Sensitivity: 50 pg/mL