DC (Deoxycholate) ELISA Kit

CAT: 0855-ELK8267-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK8267-01Size:48 Tests
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Deoxycholic Acid; Cholanoic Acid; 3a,12a-dihydroxy-5b-cholanate
Reactivity
General
Field of Research
Endocrinology
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
1000 ng/mL
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 ng/mL
Sensitivity
4.98 ng/mL

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