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Human SNCb (Synuclein beta) ELISA Kit

CAT: 0855-ELK8964-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8964-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNCb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Beta synuclein; SNCB; synuclein beta
UniProt
Q16143
Reactivity
Human
Field of Research
Signal transduction; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNCb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNCb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNCb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNCb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, cerebrospinal fluid and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
7.1 pg/mL

UniProtKB · Q16143

Beta-synuclein

SYUB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q16143
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SNCB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q6IAX7
Protein keywords

Cellular component

Cytoplasm

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Phosphoprotein

Domain

Repeat

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