Rat γH2AX (gamma H2A.X) ELISA Kit

Rat γH2AX (gamma H2A.X) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat γH2AX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat γH2AX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat γH2AX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat γH2AX in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: gamma H2A.X; γH2AX; Phospho-H2AX-S139
• Field of Research: Metabolic pathway.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 10 ng/mL
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.16-10 ng/mL
• Sensitivity: 0.059 ng/mL