SIP (Sphingosine 1 Phosphate) ELISA Kit

SIP (Sphingosine 1 Phosphate) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to S1P. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S1P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S1P in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Sphingosine 1 Phosphate
• Field of Research: Signal transduction.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 200 ng/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 3.13-200 ng/mL
• Sensitivity: 0.93 ng/mL