Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

• Gene Aliases:

  Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

• Reactivity:

  Dog

• Field of Research:

  Diagnosis of diabetes mellitus

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  1000 µg/mL

• Sample Type:

  Plasma and erythrocyte lysates

• Detection Range:

  15.63 - 1000 µg/mL

• Sensitivity:

  4.07 µg/mL

• Species:

  Dog

• CAS Number:

  7732-18-5