Anti-Cdc20(CDC20/1102), CF740 conjugate

• Description:

  Cyclins, regulatory subunits that associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CDC20, CDC20A, p55CDC, P55CDC-LSB

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CDC20

• Gene ID:

  991

• NCBI Gene ID:

  524947

• UniProt:

  Q12834

• Cellular Locus:

  Cytoplasmic|Nucleus

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human Cdc20 protein

• Target Antigen:

  Cdc20

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  CDC20/1102

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cell cycle

• Positive Control:

  Ramos or HeLa cells. Tonsil or gastric carcinoma.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Immunohistology formalin-fixed 2-4 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years