Recombinant Mouse NT-proBNP Protein(Trx Tag)

Recombinant Mouse NT-proBNP Protein(Trx Tag)

• Background: Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from the ventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF.
• Abbreviation: NT-proBNP
• Synonyms: B-Type Natriuretic Peptide;GC-B;Natriuretic Peptide Precursor B;NT-Pro-BNP;Nppb
• CAS Number: 9000-83-3
• UniProt: P40753
• Accession Number: P40753
• Host: E.coli
• Origin Species: Mouse
• Tag: N-Trx
• Sequence: Ser26-Arg76
• Field of Research: Cardiovascular;Metabolism
• Endotoxin: < 10 EU/mg of the protein as determined by the LAL method
• Purity: > 95% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
• Calculated Molecular Weight: 25.5 kDa
• Observed Molecular Weight: 30 kDa