Recombinant Mouse NT-ProBNP Protein (Trx Tag)

• Background:

  Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

• Abbreviation:

  NT-ProBNP

• Synonyms:

  B-Type Natriuretic Peptide; GC-B; Natriuretic Peptide Precursor B; NT-Pro-BNP; Nppb

• UniProt:

  P40753

• Accession Number:

  P40753

• Expression System:

  E.coli

• Tag:

  N-Trx

• Sequence:

  Ser26-Arg76

• Field of Research:

  Cardiovascular; Signal Transduction; Tags and Cell Markers; Cell Biology

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  25.6 kDa

• Observed Molecular Weight:

  22 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse