Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122)

• Background:

  It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike S1 protein. As verified in competitive ELISA-based and pseudovirus-based neutralization assay, this chimeric monoclonal can potently neutralize all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2) and Omicron (B.1.1.529) .

• Host:

  HEK293

• Origin Species:

  Mouse

• Target:

  Spike RBD

• Conjugation:

  Unconjugated

• Tag:

  Human IgG1 | Human Kappa

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.

• Purity:

  95%

• Bioactivity:

  Detection of SARS-CoV-2 S protein RBD by Sandwich ELISA Assay.
  Immobilized Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Cat. No. S1N-M122) at 2 μg/mL (100 μL/well) can bind Spike RBD (Cat. No. SPD-C52H1). And then add Biotinylated Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Cat. No.S1N-M13L3) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.05 ng/mL (Routinely tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  1mg*1

• Species:

  Mouse

• CAS Number:

  9007-83-4