Anti-CD6 (Negative Marker of T-regulatory Cells)(SPV-L14), CF594 conjugate

• Description:

  CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

• Synonyms:

  T12; TP120

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD6

• Gene ID:

  923

• NCBI Gene ID:

  744366

• UniProt:

  P30203

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse

• Immunogen:

  CD8 cytotoxic T-cell clone

• Target Antigen:

  CD6

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  SPV-L14

• Conjugation:

  CF594

• Source:

  Animal

• Applications:

  Flow (verified) | Flow, surface (published) | Functional studies (published) | IF (published) | IF (verified)

• Validated Applications:

  FC, FC, IF, IF

• Field of Research:

  Immunology

• Positive Control:

  CCRF-CEM, Jurkat cells, Tonsil

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  90-130 kDa

• Additionnal Information:

  Flow cytometry: 1-2 ug/mL|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1987) 84: 3861-3865. (Flow, surface; IF) J Inv Dermatol (1994) 102(6): 833-837. (Flow, surface) J Immunol (2006) 177:1152-1159. (functional studies) Nat Immunol (2014) 15(4): 384-392. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years