Anti-TACSTD2 / TROP2 (Epithelial Marker) (TACSTD2/2151), CF740 conjugate

• Description:

  TACSTD2 is a cell surface glycoprotein receptor. It is a single pass type I membrane protein containing one thyroglobulin type-1 domain, an epidermal growth factor-like repeat, a phosphatidylinositol binding site and tyrosine phosphorylation sites near the C-terminus. It plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. It is also highly expressed in several types of tumors and is involved in regulating the growth of carcinoma cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Cell surface glycoprotein Trop-2; Membrane Component Chromosome 1, Surface Marker 1 (M1S1); Pancreatic Carcinoma Marker Protein GA733-1; TROP2; Tumor-Associated Calcium Signal Transducer 2 (TACSTD2)

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TACSTD2

• Gene ID:

  4070

• NCBI Gene ID:

  23582

• UniProt:

  P09758

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human TACSTD2 protein (around aa 31-274) (exact sequence is proprietary)

• Target Antigen:

  TACSTD2 | TROP2

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  TACSTD2/2151

• Conjugation:

  CF740

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Signal transduction

• Positive Control:

  HT29 cells. Breast or Colon Carcinoma

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  40 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years