Anti-CD100(133-1C6)

• Description:

  CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.

• Synonyms:

  CD100; Semaphorin-4D; SEMA4D

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SEMA4D

• Gene ID:

  10507

• NCBI Gene ID:

  494406

• UniProt:

  Q92854

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Baboon, Human

• Immunogen:

  PHA stimulated human peripheral blood lymphocytes.

• Target Antigen:

  CD100 | Semaphorin-4D

• Clonality:

  Monoclonal

• Isotype:

  IgM κ

• Clone:

  133-1C6

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published) | ELISA (published)

• Validated Applications:

  FC, ELISA

• Field of Research:

  Immunology, Neuroscience, Signal transduction

• Positive Control:

  Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  50 kDa (Monomer)

• Additionnal Information:

  Immunofluorescence: 0.5-1 ug/mL|Does not react with horse, others not tested|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Frontiers Immunol (2017) 8: 765. (functional studies) Frontiers Immunol (2021) 12:240. (ELISA) Paterson J., Ailles L.E. (2018) High Throughput Flow for Cell Surface Profiling. In: Hawley T., Hawley R. (eds) Flow Protocols. Methods in Molecular Biology, vol 1678. Humana Press, New York, NY. (Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4