Anti-CD10(CB-CALLA), CF640R conjugate

• Description:

  CD10 (also known as Common Acute Lymphocytic Leukemia Antigen (CALLA)) is a type II transmembrane protein. It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD10; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen (CALLA); Enkephalinase; gp100; Membrane metalloendopeptidase; Neutral endopeptidase

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  MME

• Gene ID:

  4311

• NCBI Gene ID:

  307734

• UniProt:

  P08473

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human CD10

• Target Antigen:

  CD10

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  CB-CALLA

• Conjugation:

  CF640R

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | Flow, surface (published) | IF (verified)

• Validated Applications:

  FC, FC, IF

• Field of Research:

  Immunology, Proteases

• Positive Control:

  Tonsil, small intestine, kidney

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  100 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Immunology (2011) 134: 419-433. (Flow, surface) Blood (2015) 125(20): 3144-3152. (Flow, surface)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years