Anti-GITR / Tnfrsf18 (Mouse)(DTA-1), CF647 conjugate

• Description:

  GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25 CD4 Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-B activation, and abrogate the inhibitory function ofCD25 CD4 Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.

• Synonyms:

  Activation-inducible TNF-related ligand; AITRL; GITRL; Glucocorticoid induced TNFR related protein ligand; Glucocorticoid-induced TNF-related ligand; hGITRL; TL6; TNFSF18; Tumor necrosis factor (ligand) superfamily member 18

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  TNFRSF18

• Gene ID:

  21936

• NCBI Gene ID:

  3180

• UniProt:

  O35714

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse

• Immunogen:

  Mouse CD25 CD4 T cells

• Target Antigen:

  GITR | Tnfrsf18

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  DTA-1

• Conjugation:

  CF647

• Source:

  Animal

• Applications:

  Flow, surface (published) | Functional studies (published)

• Validated Applications:

  FC, Functional assays

• Field of Research:

  Immunology, Inflammation

• Positive Control:

  CD25 CD4 Tregs.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  66-70 kDa (Homodimer)

• Additionnal Information:

  Flow cytometry: 0.5-1 ug/million cells in 0.1 mL|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Clin Cancer Res (2013) 19(5): 1044-53. (functional studies) J Immunol (2019) 203:864-872. (Flow)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years