Chicken anti Clostridium difficile Toxin A

Chicken anti Clostridium difficile Toxin A

• CAS Number: 9007-83-4
• UniProt: P16154
• Host: Chicken
• Conjugation: Affinity Purified
• Type: Primary Antibodies
• Source: Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.
• Applications: WB, ELISA
• Field of Research: Microbiology, Toxic Compounds, Infectious Agents and Disease
• Purification Method: Egg-yolk derived IgY, affinity purified
• Assay Principle: This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 µg of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 µg/mL coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.
• Concentration: See vial for Concentration
• Form: Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Bochner, B and X. Lei. 2015. Compositions and Methods to Inactivate and/or Reduce Production of Microbial Toxins. United States Patent Application 2015 0374786._x000D_ 2. Tussey, L., Song, L. and S. Umlauf. 2016. Clostridium difficile Compositions and Methods of Use. United States Patent Application 2016016667.
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles