Chicken anti Clostridium difficile Toxin A

• UniProt:

  P16154

• Host:

  Chicken

• Conjugation:

  Affinity Purified

• Type:

  Primary Antibodies

• Source:

  Chickens were immunized with highly purified Toxin A purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin A was immunoaffinity purified using the immunogen immobilized on a solid support.

• Applications:

  WB, ELISA

• Field of Research:

  Microbiology, Toxic Compounds, Infectious Agents and Disease

• Purification Method:

  Egg-yolk derived IgY, affinity purified

• Assay Principle:

  This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:4000 dilution of the IgY was an effective probe for 1 µg of Toxin A. By Direct ELISA, the half-point titre was 1:20,000 using a 2 µg/mL coating of Toxin A. Optimal working dilutions should be determined for your particular assay conditions.

• Concentration:

  See vial for Concentration

• Form:

  Phosphate buffered saline‚ pH 7,2 with 0,075% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Bochner, B and X. Lei. 2015. Compositions and Methods to Inactivate and/or Reduce Production of Microbial Toxins. United States Patent Application 2015 0374786._x000D_ 2. Tussey, L., Song, L. and S. Umlauf. 2016. Clostridium difficile Compositions and Methods of Use. United States Patent Application 2016016667.

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• CAS Number:

  9007-83-4