Anti-Arginase 1 (Hepatocellular Carcinoma Marker) (ARG1/1125) , CF640R conjugate

• Description:

  This antibody recognizes a protein of 35-38 kDa, which is identified as Arginase 1 (ARG1). Arginase is a manganese metallo-enzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibodies to Arginase 1 label hepatocytes in normal tissues and granulocytes in peripheral blood. Arginase 1 is a sensitive and specific marker for identification of hepatocellular carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Arginase 1; ARG1; liver-type arginase; type I arginase

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ARG1

• Gene ID:

  383

• NCBI Gene ID:

  440934

• UniProt:

  P05089

• Cellular Locus:

  Cytoplasmic

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human ARG1 protein fragment (around aa11-97) (exact sequence is proprietary)

• Target Antigen:

  Arginase 1

• Clonality:

  Monoclonal

• Isotype:

  IgG3 κ

• Clone:

  ARG1/1125

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  IHC, FFPE, WB

• Field of Research:

  Cancer, Metabolism

• Positive Control:

  293Tcells. Hepatocellular Carcinoma (HCC).

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  35-38 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 2-4 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 1-2 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4