DPA

• Description:

  Dipicrylamine (DPA) is a nonfluorescent anionic membrane dye. With its absorbance in the blue region of the visible spectrum, DPA can be used as a FRET quencher for a variety of fluorescent donor dyes. When applied to cells, DPA localizes at the interface of cytoplasmic membrane and aqueous phase in a manner sensitive to membrane potential change. A recent application of DPA is to measure cell membrane potential change by combining with the fluorescent donor dye DiOC16. The DiO/DPA system has been reported to produce a fluorescence signal change of gt;56% in HEK-293 cells and gt;25% in neuronal cultures and brain slices per 100 mV membrane potential change. Please also see the DiO/DPA Membrane Potential Detection Kit.

  Yellow solid soluble in DMSO at gt;30mg/mL

  • lamba;abs (MeOH) = 406 nm
  • Store at 4°C
  • MWt: 439.21
  • [131-73-7]

• UNSPSC:

  41116113

• UNSPSC Description:

  Cytology reagents or solutions or stains

• Source:

  Synthetic

• Storage Conditions:

  4°C; Store with desiccant; Protect from light

• Shelf Life:

  12 months

• CAS Number:

  131-73-7