NucView® 488 and MitoView™ 633 Apoptosis Assay Kit (100 assays)

NucView® 488 and MitoView™ 633 Apoptosis Assay Kit (100 assays)

• Description: This kit contains the NucView®488 Caspase-3 Substrate and the MitoView trade;633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The bi-functional NucView®488 Caspase-3 Substrate is able to detect both intracellular caspase-3 and at the same time stain the cell nucleus, which is known to undergo morphological changes during apoptosis. MitoView trade;633 is a far-red fluorescent mitochondrial dye. The staining is dependent upon the mitochondrial membrane potential; thus, apoptotic cells exhibit a much lower MitoView trade;633 dye staining compared to healthy cells. Furthermore, the spectral separation of these two dyes minimizes fluorescence overlap.
  Note: The optimal detection settings for MitoView trade; 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes.
  See our full selection of NucView® substrates and kits. We also offer MitoView trade; Dyes separately.
  NucView® enzyme substrate technology is covered by U.S. Patents. Cy dye is a registered trademark of GE Healthcare.
• UNSPSC: 41116113
• UNSPSC Description: Cytology reagents or solutions or stains
• Source: Synthetic
• Storage Conditions: 4°C; Protect from light
• Shelf Life: 6 months