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CaspSCREEN™ Flow Cytometric Apoptosis Detection Kit

CAT: 0026-K200-25Size: 25 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K200-25Size:25 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Activation of members of caspase-family proteases plays a key role in apoptosis. The CaspSCREENTM Flow Cytometric Apoptosis Detection Kit provides a convenient means for detecting activation of caspases by flow cytometry in living cells. The assay is based on the cleavage of (aspartyl)2-Rhodamine 110 (D₂R), a reported substrate for members of caspase family proteases. The caspase substrate D₂R is non-fluorescent, however, upon cleavage of the substrate by cellular caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at excitation of 488 nm and emission of 530 nm.
Features & Benefits
• Simple one-step procedure; takes only 20-30 minutes • Fast and convenient • D₂R is more cell-permeable than other fluorometric caspase substrates
Shipping Conditions
gel pack
Storage Conditions
-20°C
Shelf Life
6-12 months

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