TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07]

TNF alpha Recombinant Rabbit Monoclonal Antibody [PS01-07]

• Description: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.
• Product Name Alternative: APC1 antibody APC1 protein antibody Cachectin antibody DIF antibody Differentiation inducing factor antibody Macrophage cytotoxic factor antibody Tnf antibody TNF superfamily member 2 antibody TNF superfamily, member 2 antibody TNF, macrophage derived antibody TNF, monocyte derived antibody TNF-a antibody TNF-alpha antibody TNFA antibody TNFA_HUMAN antibody TNFSF2 antibody Tumor necrosis factor (TNF superfamily member 2) antibody Tumor necrosis factor alpha antibody Tumor necrosis factor antibody Tumor necrosis factor ligand superfamily member 2 antibody Tumor Necrosis Factor, Membrane Form antibody Tumor necrosis factor, soluble form antibody
• Abbreviation: Tumor necrosis factor Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2 (TNF-a) Tumor necrosis factor, membrane form Alternative names: N-terminal fragment (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form TNF TNFA, TNFSF2
• CAS Number: 9000-83-3
• Swiss Prot: P01375 Human, P06804 Mouse
• Cellular Locus: Cell membrane, Secreted.
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein within full length protein.
• Isotype: IgG
• Clone: PS01-07
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB
• Positive Control: THP-1 treated with 500ng/mL LPS for 24 hours then add 300ng/mL BFA for 20 hours cell lysate, RAW264.7 treated with 100ng/mL LPS for 7 hours add 300ng/mL BFA for 3 hours cell lysate.
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 26 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes