Recombinant Mouse PDGF-BB

• Product Name Alternative:

  PDGFBB, PDGF-BB

• UniProt:

  AAH53430.1

• Expression Region:

  82-190

• Host:

  E.Coli

• Tag:

  C-terminal His Tag

• Applications:

  Western Blot, ELISA

• Endotoxin:

  < 1 EU/µg as determined by LAL test.

• Purity:

  Greater than 95% as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using Balb/c3T3 mouse fibroblast cells. The ED50 for this effect is 38.7 ng/ml.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in 4 mM HCl to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  15 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.

• Organism Species:

  Mouse