PDGF-BB Recombinant

• Description:

  Recombinant PDGF-BB is a disulfide-linked  homodimeric protein consisting of a two 109 amino acid residue subunits, and migrates as an approximately 32 kDa protein under non-reducing and 16 kDa under reducing conditions. Optimized DNA sequence encoding human PDGF-BB mature chain was expressed inSaccharomyces cerevisiaecells.

• UniProt:

  P01127

• Endotoxin:

  Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.

• Purity:

  ≥ 98% by SDS-PAGE and HPLC analysis.

• Format:

  Lyophilized protein

• Reconstitution:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.

• Shipping Conditions:

  -20°C or-80°C

• Storage Conditions:

  ≥2 years (lyophilized) at -20°C.

• Formulation:

  Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.