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E-Cadherin

CAT: 0013-GTR18398517-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398517-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
E-Cadherin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM) ; Uvomorulin (UVO)
UniProt
P12830
Reactivity
Human
Immunogen
Recombinant full-length human E-Cadherin protein
Clonality
Monoclonal
Clone
MSVA-035R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: In the liver, at least a moderate membranous staining should be seen in hepatocytes while bile duct epithelia stain strongly. In the colon, only epithelium should display a strong membranous staining reaction. Negative Control: Tonsil or appendix: No staining reaction must be seen in stromal cells such as lymphocytes, plasma cells, smooth muscle cells or endothelial cells. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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