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Supreme TC Grade Agar

CAT: 0644-CN-A060-1KGSize: 1 KgDry Ice: NoHazardous: No
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CAT#:0644-CN-A060-1KGSize:1 Kg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Solidifying agents play a vital role in tissue culture media, providing essential support for the optimal growth and development of plants when incorporated into the medium. Additionally, the inclusion of solidifying agents helps ensure the even distribution of nutrients, hormones, and other crucial elements throughout the culture medium. The two most common solidifying agents used in the process are agar and gellan gum. Agar, derived from seaweed, is a polysaccharide. It imparts a gel-like texture to the medium, facilitating the adherence, growth, and differentiation of plant cells.
CAS Number
9002-18-0
Synonyms
Gelose, Agar Agar Agar
HS Code
1302.31
Applications
Agar is used as microbial solid culturing media. The substance is often used in doses ranging between 0.5 to 1.0% (w/v) to solidify plant tissue culture media. Agar is an essential ingredient used for in vitro root development.
Assay Protocol
Follow the given steps to prepare 1 liter of tissue culture media using PCT’s Supreme TC Agar: Take a beaker and add 800 ml of distilled water to it. To the water add 4.54 grams of PCT’s MS Media (or alternative medium based on your plant), 30 grams of sugar, and PPM™ (generally 1-2 ml/L). Make up the volume of the media to 1000ml. Adjust the pH of the media between 5.4-5.8. Add 6-8 grams of PCT’s Supreme Tissue Culture Grade Agar to the media and keep stirring. Autoclave the medium for 20 minutes at 15 psi and 121°C.
Capacity
Available in different weights of 100 gm, 500 gm, 1 Kg, and 5 Kg
Format
Cylindrical container
Material
Powder
Color
Light beige
Solubility
Partially soluble in cold water and completely soluble in hot water
Features & Benefits
Easy to use Acts as a solid support for the growth and development of explants Allows the absorption of nutrients by the plant cells Cost-effective
Storage Conditions
Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from combustible substances.
Storage Temperature
20-22 ºC
Shelf Life
See expiry date on the bottle
Strength
980 g/cm2

Chemical Information

Agar

A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.

CAS Number9002-18-0
PubChem CID71571511
IUPAC Name(2R,3S,4S,5R)-2-(hydroxymethyl)-6-[[(4R,5S)-4-hydroxy-3-methyl-2,6-dioxabicyclo[3.2.1]octan-8-yl]oxy]-4-methoxyoxane-3,5-diol
Molecular FormulaC14H24O9
Molecular Weight336.33
XLogP-2.2
Topological Polar Surface Area127
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count9
Rotatable Bond Count4
Heavy Atom Count23
Formal Charge0
Complexity408
SMILES
CC1[C@H]([C@H]2C(C(O1)CO2)OC3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)OC)O)O
InChI
InChI=1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
InChIKey
GYYDPBCUIJTIBM-DYOGSRDZSA-N
Chemical Structure
2D Structure
2D structure of Agar
CAS: 9002-18-0
CID: 71571511
Formula: C14H24O9
MW: 336.33
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight336.33
XLogP3-2.2
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count9
Rotatable Bond Count4
Exact Mass336.14203234
Monoisotopic Mass336.14203234
Topological Polar Surface Area127 Ų
Heavy Atom Count23
Formal Charge0
Complexity408
Isotope Atom Count0
Defined Atom Stereocenter Count6
Undefined Atom Stereocenter Count4
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes
Certificate of Analysis
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Product MSDS
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