One-step TUNEL Flow Cytometry Apoptosis Kit (Green, Elab Fluor® 488)

• Type:

  Apoptosis / DNA Fragmentation

• Detection Method:

  Fluorometric method; Flow cytometry

• Assay Principle:

  When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.

• Assay Performance Time:

  2.5 - 3 Hours

• Sample Type:

  Cell samples

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.12002

• Detection Instrument:

  Flow Cytometer