One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor® 555)

• Type:

  Apoptosis / DNA Fragmentation

• Detection Method:

  Fluorometric method

• Assay Principle:

  When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

• Assay Performance Time:

  3 Hours

• Sample Type:

  Paraffin section; frozen section; cell slide

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.science.org/doi/abs/10.1126/sciimmunol.adc9081

• Citation 02:

  https://aacrjournals.org/cancerres/article/doi/10.1158/0008-5472.can-23-2491/735128

• Citation 03:

  https://www.nature.com/articles/s41401-023-01218-z

• Citation 04:

  https://biosignaling.biomedcentral.com/articles/10.1186/s12964-024-01479-z

• Citation 05:

  https://www.ahajournals.org/doi/abs/10.1161/atvbaha.123.319119

• Citation 06:

  https://www.nature.com/articles/s41420-023-01740-3

• Citation 07:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/glia.24449

• Citation 08:

  https://journals.physiology.org/doi/abs/10.1152/ajpcell.00348.2023

• Citation 09:

  https://www.mdpi.com/1422-0067/24/13/11210

• Citation 10:

  https://www.sciencedirect.com/science/article/pii/s1567576924000158

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/s221345302400226x

• Citation 12:

  https://iovs.arvojournals.org/article.aspx?articleid=2793368

• Detection Instrument:

  Fluorescence Microscope