Caspase 3/7 Activity Assay Kit (Colorimetric Method)

• Type:

  Apoptosis / Caspase

• Detection Method:

  Colorimetric method

• Assay Principle:

  Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline) . The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.

• Assay Performance Time:

  4 - 6 Hours

• Sample Type:

  Cell and tissue samples

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.tandfonline.com/doi/abs/10.2147/ijn.s417928

• Citation 02:

  https://www.sciencedirect.com/science/article/pii/s0925521423003162

• Citation 03:

  https://www.sciencedirect.com/science/article/pii/s0925521423001369

• Citation 04:

  https://www.sciencedirect.com/science/article/pii/s0141813024082667

• Citation 05:

  https://www.mdpi.com/2073-4409/12/23/2734

• Citation 06:

  https://www.mdpi.com/2072-6643/14/21/4605

• Citation 07:

  https://www.sciencedirect.com/science/article/pii/s0925521422002678

• Citation 08:

  https://www.sciencedirect.com/science/article/pii/s0928098722000847

• Citation 09:

  https://www.frontiersin.org/articles/10.3389/fphar.2021.735165/full

• Citation 10:

  https://www.mdpi.com/1138736

• Citation 11:

  https://www.sciencedirect.com/science/article/pii/s0300483x22002621

• Citation 12:

  https://www.sciencedirect.com/science/article/pii/s1773224724008281

• Detection Instrument:

  Spectrophotometer

• Result Type:

  Qualitative or quantitative