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TdT antibody

CAT: 0013-GTR18398462-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398462-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TdT antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Deoxynucleotidyltransferase terminal; DNTT; Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase; TDT; Terminal addition enzyme; Terminal deoxynucleotidyltransferase; Terminal deoxyribonucleotidyltransferase
UniProt
P04053
Reactivity
Human
Clonality
Monoclonal
Clone
MSVA-453R
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
1:75-1:150
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus. Negative Control: Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. ; Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative. Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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