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Vimentin antibody

CAT: 0013-GTR18398461-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398461-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
VIM
UniProt
P08670
Reactivity
Human
Immunogen
A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-458R
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining. Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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