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CTLA-4 antibody

CAT: 0013-GTR18398439-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398439-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CTLA-4 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
ALPS5; CD152; Celiac disease 3 (CELIAC3) ; Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4) ; GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)
UniProt
P16410
Reactivity
Human
Clonality
Monoclonal
Clone
MSVA-152R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A moderate strong membranous and cytoplasmic CTLA4 immunostaining should be seen in a subset of T-lymphocytes with strongest staining in follicular T-helper cells of germinal centres. Negative Control: Tonsil: The majority of lymphocytes in germinal centres and all epithelial cells should stain negative. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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