Products for Research Use Only

Periostin antibody

CAT: 0013-GTR18398413-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398413-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Periostin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Fasciclin-I like; Osteoblast specific factor 2 (OSF2) ; PDLPOSTN; Periodontal ligament specific periostin; Periostin isoform thy2 / th4 / thy6 / thy8; Periostin osteoblast specific factor; PN; POSTN
UniProt
Q15063
Reactivity
Human
Immunogen
Recombinant fragment (around aa193-326) of human POSTN protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-649M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Skin: A strong periostin immunostaining should be seen in the skin, forming a subepithelial band of variable thickness. Negative Control: Skin: Periostin immunostaining should be lacking in epithelial cells. Cellular Localization: Extracellular space Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa