Products for Research Use Only
RegisterLogin

Occludin antibody

CAT: 0013-GTR18398411-01Size: 0.1 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18398411-01Size:0.1 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin
UniProt
Q16625
Reactivity
Human
Immunogen
Ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-415M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes. Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa

Related Products

CatalogName
GTR19020309-01Occludin
GTR19013345-01Occludin
GTR19013023-01Occludin
GTR19013066-01Occludin
GTR19013433-01Occludin
GTR18294666-01Occludin polyclonal antibody
GTR18045789-01Occludin polyclonal antibody
GTR18316745-01OCLN Antibody / Occludin
GTR18869540-01Mouse Occludin (Occludin) ELISA Kit
GTR18874654-01Rat Occludin (Occludin) ELISA Kit

Alternative Products

CatalogName
GWB-2C5554Occludin, Antibody
MBS8583702-01Occludin Antibody

Popular Products