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Uroplakin 1A antibody

CAT: 0013-GTR18398410-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398410-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
UP1A; UPIA; UPKA; TSPAN21
UniProt
O00322
Reactivity
Human
Immunogen
Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-735M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium) . Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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